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Vascul Pharmacol, 2018

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179 mg/day) was associated with a 28% lower risk of fatty liver disease in normal-weight women, but no association was found in overweight or obese women or in men

[Products in use] Extracellular LDH : Cytotoxicity LDH Assay Kit-WST (Product code: CK12) Phosphatidylserine: Annexin V Apoptosis Plate Assay KitProduct code: AD12 Cell viability : Cell Counting Kit-8 (Product code: CK04) Intracellular Fe 2+ : FerroOrange (Product cose: F374) *Normalized with Hoechst 33342 fluorescence intensity Lipid peroxidation : Lipid Peroxidation Probe -BDP 581/591 C11- (Product code: L267) [Experimental conditions] Cell type: HepG2 cell(210 4 cells/well) Drugs: Staurosporin(5 mol/l), Erastin(25 mol/l), Erastin+RSL3(both 25 mol/l) *Diluted in serum-free medium Experimental Example: Evaluation using apoptosis inhibitors Experimental Example: Evaluation using Erastin-treated cells Changes in extracellular ATP release, cell viability, extracellular LDH release, phosphatidylserine, and intracellular Fe2+ were evaluated in HeLa cells treated with various concentrations of Erastin, a ferotosis inducer, for 24 hours

van Wijck K, Lenaerts K, Grootjans J, Wijnands KA, Poeze M, van Loon LJ, Dejong CH, Buurman WA: Physiology and pathophysiology of splanchnic hypoperfusion and intestinal injury during exercise: strategies for evaluation and prevention