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Grossman HB

Within the liver, macrophages are called Kupffer cells

Of note, data on the role of circadian machinery in anti-tumor immunity suggest a possible role of circadian machinery in the development of cold tumors through the accumulation of immune-suppressive cells in the microenvironment and through the regulation of the expression of PDL1 and CTLA4, two major targets of the current immunotherapy 126

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Briefly, harvested cells were suspended in a hypertonic solution of 30 mM Tris, 20% w/v sucrose, 1 mM EDTA, pH 8 (25 mL) and incubated for 30 min at 4 C