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Tumor spheroid viability assay K1 cells were incubated to form spheroids (~ 7 days) in ultra-low binding 96-well plates for viability assay and in ultra-low binding 24-well plate for imaging with PI staining

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The expression of cat induced by different concentrations of formaldehyde was tested to verify whether the amplifier could solve the problem of leakage expression, and the growth curve was measured to verify whether it could relieve the expression pressure of strains

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Br J Psychiatry 220:219228