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(vi) Resuspend the cells in 1 ml of ice-cold extraction buffer (0.1% Triton-X and 0.6% sulfosalicylic acid in KPE) and homogenize cells with a Teflon pestle

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However, both aging and cancer are characterized by a series of partially overlapping hallmarks. The equivalence or antagonism between aging-associated deregulated nutrient-sensing and cancer-relevant alterations of cellular metabolism is complex