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The impact of this deficiency on neonatal health especially in hyperbilirubinemia and kernicterus has led many countries with moderate to high prevalence to implement universal screening

Insulin-like growth factor-1 (IGF-1) is known as a neurotrophic factor and is studied as a potential therapeutic approach in different disorders of the brain

The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat

Raychaudhuri et al., 2018)

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