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Antibodies used: anti-IL-6 (1:50, Proteintech, 21865-1-AP), anti-occludin (1:100, Proteintech, 13409-1-AP), anti-Muc2 (1:100, Proteintech, 27675-1-AP), anti-Ki67 (1:250, Abcam, ab16667), anti-LGR5 (1:400, Affinity, DF2816) and anti-HMGCS2 (1:100, ABclonal, A14244)

These reactive molecules include reactive oxygen species , commonly abbreviated as ROS

Nootropic peptides are specifically designed to permeate this barrier effectively and exert their influence on the brain's neurochemistry [7, 16]

Studies show that silymarin can stimulate the synthesis of glutathione in the liver and protect it from degradation an important mechanism for antioxidant liver protection

(1) Mechanism of action: Pyridostigmine is a reversible acetylcholinesterase inhibitor that prevents acetylcholine (ACh) breakdown at the neuromuscular junction, thereby increasing neurotransmitter availability and enhancing neuromuscular transmission