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In a method for fipronil and its metabolites, the limits of detection (LODs) have been reported as 0.0005 mg/L for the metabolites and 0.0003 mg/L for fipronil, while the LOQs were established at 0.002 mg/kg for all compounds across maize grain, maize stem, and soil, which is below the established tolerance levels in the USA and European Union (Spanjers et al., 2017)

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The average rotation value was calculated among all microtubule segments in a single image and used as a reference axis