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GPx3 mRNA is also detected in other tissues, such as liver, heart, breast, lung 4 , skeletal muscle, pancreas, brain 5 , mature absorptive epithelial cells 6 and thyroid 7 , from where Gpx3 is secreted into the surrounding extracellular environment

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Larger, placebo-controlled, biomarker-guided trials are needed to clarify efficacy, mechanisms, and optimal microbial strains

To address these limitations, the current protocol employs a Tiron/ferrous ion (Fe 2+ ) reagent, which eliminates the requirement for protein precipitation and effectively resolves the issues associated with earlier methods