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Stroissnigg, H

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They admittedly speculate that the "intensity and duration of the bottleneck(s) leading to breed formation may have been extreme in cKCs relative to in most other breeds." The "bottlenecks" they refer to include (a) development of a brachycephalic variety of the King Charles spaniel, presumably in the late 1800s, followed by "the old, more long-nosed type was restored by a small breeding initiative starting in the 1920's"

Protein analysis of biopsy samples Muscle biopsy samples were allocated for protein determination by western blot analysis with antibodies (New England BioLabs, Mississauga, ON, Canada) against total and the phosphorylated protein targets: mTOR, phospho-mTOR (Ser2448), p70-S6K, phospho-p70-S6K (Thr389), 4E-BP1, and phospho-4E-BP1- (Thr37/46)

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