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5.2 Effect of IF on ceramides levels, ETC and ROS generation Excessive glucose flux has been described as increasing the activity of the mitochondrial ETC, leading to ROS overproduction (Hallan and Sharma, 2016

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Prepare Diluent: Pull an amount of air into the mixing syringe equal to the amount of diluent you plan to add (for EXAMPLE: 1 mL) to create negative pressure

To facilitate insertion it is recommended you moisten the product by passing it under a stream of cool water or use a personal lubricant

The following gradient was used for peptide separation: from 2% B to 25% B over 25 min to 35% B over 5 min to 95% B over 1 min followed by 5 min at 95% B to 2% B over 1 min followed by 3 min at 2% B