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That is, when glutamate binds to the AMPA receptor, the shape of the receptor protein changes, opening a channel for Na + ions

The relative expression of target genes was calculated using the 2e(-Ct) method ( ELISA To access the intracellular protein levels of CCL3 and IL-1B, cell precipitates of aliquots from endpoints A and B were homogenized in 1X PBS, diluted in Milli-Q H 2 O 1:2 and the cells disrupted by mechanical lysis in the TissueLyser (Qiagen) for 2 minutes at 30 Hz and three freeze/thaw cycles (20 to -20C)

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To purify 6His-tagged uS10, the method described above was performed

Proliferation and migration of the endothelial cells is necessary for angiogenesis to happen