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After 3 h of OGD treatment in HUVECs, WB results showed that the expression of Ki-67 and Lamin B decreased, while the expression of P53, P21, P16, and -H2AX significantly increased (Figure 3A)

Bulk RNA sequencing revealed that gene expression changes were most pronounced in the MBH, where RAMP1/3 KO mice treated with sCT showed extensive differential expression of over 1,700 genes, while WT mice exhibited comparatively minor transcriptional shifts