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The model was characterized by immunocytochemistry and permeability for marker molecules

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Safety concerns included uncharacterized immunogenicity (the risk that injected peptides trigger immune reactions), inadequate impurity and endotoxin testing in available raw material, the unresolved free base versus acetate salt form distinction, and, for BPC-157 specifically, an angiogenesis mechanism that raises theoretical cancer-risk concerns for certain patient populations

For example, certain microbial metabolites (like SCFAs) can promote -syn aggregation in enteric neurons, as shown in one mouse study [95]