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In summary, Table 16 lists the 27 APCs that we are excepting from the 2 times rule for CY 2026 based on the criteria described earlier and a review of updated claims data for dates of service between January 1, 2024, and December 31, 2024, that were processed on or before June 30, 2025, and updated CCRs, if available

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Samples were then evaporated to dryness in Speed vac and resuspended in 500 l Buffer A (95% H 2 O, 5% CH 3 CN, 0.1% FA) with sonication and loaded on HPLC for offline high pH fractionation following conditions adapted from previous work 57

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