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2.11 Enzyme-linked immunosorbent assay (ELISA) for cytokine quantification The levels of pro-inflammatory cytokines TNF- and IL-1 in the culture supernatant of SH-SY5Y cells were quantified using ELISA kits (R&D Systems, Inc., Minneapolis, United States)

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Each bottle contains approximately 250 tablets, providing a complete system for cellular mineral support and balance based on Dr

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4) Underexplored therapeutic strategies and model limitations: Preclinical studies have relied on in vitro mesangial cell models and lack validated animal models that fully recapitulate human IgA nephropathys pathological features (e.g., glomerular IgA1 deposition and mesangial proliferation)