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CircRNAs, as endogenous miR-9 sponges, can target miR-9 to participate in the ubiquitination of HSP90, thereby regulating microglial polarization and reducing depressive-like behaviors 170

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In an effort to quantify the binding affinity, we measured complex formation throughout a 4HNE titration series, observing an apparent K D of 398 +/ 80 M ( Figure 2C and D )