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Briefly, mice were decapitated and the brain was rapidly extracted in oxygenated (95% O2 and 5% CO2) and ice-cold sucrose-based artificial cerebrospinal fluid (sucrose-aCSF) at pH 7.4 and 300 mOsm containing: 185 mM sucrose (Sigma Aldrich S9378), 25 mM NaHCO 3 (Thermo Fisher, BP328), 2.5 mM KCl (Millipore Sigma, P9333), 25 mM glucose (Millipore Sigma, G8270), 1.25 mM NaH 2 PO 4 (Sigma Aldrich S8282), 10 mM MgCl 2 (Millipore Sigma, M9272), and 0.5 mM CaCl 2 (Millipore Sigma, 223506)

Role in cell proliferation: Different studies indicate that glutathione promotes proliferation of different types of cells such as human fibroblast cells, lymphocyte, hepatocytes, mouse bone marrow cells and intestinal epithelial cells

Digestive enzymes can break down peptides before theyre absorbed

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It has sufficient composition of all essential amino acids that meet standard dietary recommendations (Thevarajah et al