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Cells were dehydrated with an ethanol series (20%, 50%, 70%, 90% and 100%) on ice for 1 min each and then 100% dry ethanol at room temperature three times for 1 min each, infiltrated with 50:50 dry ethanol:Durcupan ACM resin (Sigma-Aldrich, 44610) for 30 min and then four changes of Durcupan at 1 h each and finally embedded in a vacuum oven at 60 C for 72 h

Those focused on muscle development should also explore peptides for muscle growth for complementary approaches

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3) Transfer the sonicated solution to a new tube, centrifuge at 10000 rpm for 20-30 minutes at 4C