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The following sgRNAs for GGT1 CRISPRi were used: sgGGT1 1 (HL-ID: 3) forward: 5-CACCGGTGCCTCCCACTGTCCGCCG-3

This excitotoxicity is not limited to cortical areas but also occurs in subcortical structures like the IC, where salicylate-induced neuronal hyperactivity and oxidative injury are closely linked to NMDA receptor-mediated mechanisms (Wang et al., 2023)

Taken together, these results suggest that Ssu72 induces adipogenesis and lipogenesis in white adipocytes by GSK3/-catenin axis under HFD conditions, contributing to adipose tissue expansion during diet-induced obesity