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Fluid content of fecal pellets During the investigation period, each fecal pellet was collected and weighed first as wet weight and then again as dry weight after being placed in a 37 C oven overnight

This observation has been confirmed by molecular pharmacology studies from multiple laboratories

Evaluating the effect of black cumin on asthma-related inflammatory mediators, Koshak and the team reported that the oily TQ-rich extract promoted immune response by reducing IL-2, IL-6, and PGE2 in primary T-lymphocytes and IL-6 and PGE2 in primary monocytes [41]

Takuva S, Louwagie G, Zuma K, et al

Immunoprecipitation and co-immunoprecipitation (Co-IP) Protein was extracted from cells using lysis buffer (HEPES 50 M, NaCl 150 M, EDTA 1 mM, 1% Triton, 10% glycerol) supplemented with protease inhibitor cocktail, centrifuged at 4 C, 10000 g for 30 min