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First, approximately 9% of siRNA was released over 4 h, which is related to the sustained release of the surface-loaded siRNA ( siRNA dissociation from the nanocomplex in the cytoplasm is an important step for successful siRNA-mediated knockdown ( Efficient Cellular Uptake of AuNPs-TMC/siRNA Into Breast Cancer Cells Effective silencing of the target EGFR mRNA depends on successful internalization of NPs by cancer cells with subsequent release of siRNA in the cytoplasm (Figure 2C), after 4 h incubation, the nanocomplex was effectively internalized into the MCF-7 cells and localized inside the cytoplasm, where siRNA mediates its function by interaction with the RNA interference machinery (Figure 2D) also confirmed the successful and complete cellular uptake of the nanocomplex after 4 h incubation

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