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SetCys peptide 1 (1.32 mg, 0.881 mol, 1.2 equiv), cysteinyl peptide CILKEPVHGV-NH 2 (1.27 mg, 0.884 mol, 1.2 equiv) and peptide thioester 4 (1.14 mg, 0.735 mol, 1 equiv) were then successively dissolved in this solution (92 L) and the reaction mixture was stirred at 37 C

The pH value of the solution formed was adjusted to 7.1 ~ 7.2 using hydrochloric acid (HCl) or NaOH and then filtered the solution

Starting at lower doses and increasing gradually reduces the likelihood of systemic effects

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(Note: Discusses JHR in Lyme-like spirochetal contexts.) Brewer, J