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Western blot analysis Protein lysates were obtained by homogenizing tissues or cell pellets in sample buffer containing 62.5 mM Tris-HCl (pH 6.8), 4% SDS, 200 mM dithiothreitol (DTT), 10% glycerol, and 0.001% bromophenol blue using a tissue to buffer ratio of 1:10 (w/v), followed by boiling

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c) RT-qPCR of ALB, ACSS2, ASL, CPS1 , and OTC (zone 1) gene for Z1- and Z3-HLOs compared to freshly isolated PHH, H20 (20 um periportal hepatocytes) and H40 (40 um pericentral hepatocytes) (Data is mean SD, n = 9 independent experiments)

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