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Because your body uses and excretes B12 rather than storing unlimited amounts, consistent timing helps keep your energy and focus steady instead of peaking and crashing. Many people find that taking B12 late in the day can make them feel more alert right when they are trying to wind down, which may interfere with sleep

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Intravital imaging To test if ABT treatment influences OTA transport kinetics, intravital imaging of OTA transport in the livers of mice pre-treated with PBS or ABT was performed using a two-photon microscope (LSM MP7, Zeiss, Germany), as previously described (Hassan 2016