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Most clients report only mild discomfort during the injection

Immunofluorescence Cells grown on coverslips were fixed with cold methanol at 20 C and then incubated with the following primary antibodies: anti-LC3A/B rabbit monoclonal (1:100) (Cell Signaling Technology, Inc., New England Biolabs., UK), anti-Phospho-S6 Ribosomal Protein rabbit monoclonal (1:100) (Cell Signaling Technology), anti p53 mouse monoclonal (1:100) (Dako Cytomation, Glostrup, Denmark) and anti-Ki67 rabbit polyclonal (1:300) (Abcam, Cambridge Science Park, Cambridge, UK)

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Mass spectra were acquired using a Data Dependent Acquisition mode (DDA) with full scan spectra done in the Orbitrap while fragments ions were generated by Higher energy Collision-induced Dissociation (HCD) and measured in the linear ion trap

Dimethyl fumarate induces ferroptosis and impairs NF-kappaB/STAT3 signaling in DLBCL