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The analyses of 4MSOB ITC and its conjugates were conducted using an Agilent HP1260 Series instrument coupled to an API5000 tandem mass spectrometer (AB Sciex, Darmstadt, Germany) by loading extracted samples onto an Agilent Zorbax Eclipse XDB-C18 column (50 4.6 mm 1.8 m, Agilent Technologies, Waldbronn, Germany) with mobile phase A (0.05% formic acid in milliQ water) and mobile phase B (acetonitrile)

BMC Public Health 2012;12:394404

Plasma protein binding of the drug with inhibited biotransformation

The plasma AOD-9604 half life refers to its clearance from the bloodstream, while its biological half-life might encompass the persistence of its downstream cellular effects

In simple terms, the molecules become less likely to stick together, which can help the peptide move into a clearer and more uniform solution