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Berberine Was Neuroprotective against an In Vitro Model of Brain Ischemia: Survival and Apoptosis Pathways Involved

Reverse transcription and real-time quantitative PCR (Q-PCR) Total RNA was isolated from frozen liver pieces using Tri-Reagent (Molecular Research Center, Inc.) according to manufacturers protocol

thus, for example, assay screens for up to about 6,000-20,000 different compounds are possible using the described integrated systems

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Patients did not consume any meals or drinksexcept pure waterat least 2 h before the saliva collection