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After 5 min at room temperature, 30 l/well of 1 N sulfuric acid was added and the A 450 of each well was read on a Molecular Devices SpectraMax M2 plate reader

Effect of nitric oxide on hyperpolarization-activated current in substantia gelatinosa neurons of rats

Median GC was 117.53 (IQR = 91.31150.0) in the 2nd trimester and 110.22 (IQR = 83.10146.13) in the 3rd trimester, also with no significant difference (p = 0.46)