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Cells were then harvested by centrifugation at 1,000 g for 3 min at room temperature and resuspended in 100 mM MES/Tris buffer, pH 6, to an OD 600 of 7.5

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Microglia have been classically divided into two activation phenotypes, M1 (classical activation phenotype) and M2 (alternative activation phenotype) (Luo and Chen, 2012)

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