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Validity and reproducibility of arterial pulse wave velocity measurement using new device with oscillometric technique: A pilot study

Discussion The genetic alterations such as mutations and SNPs in many regulatory genes including TP53, KRAS, DDR2, KLK3 etc

4.Enzyme marker inactivation or substrate failureMix the enzyme conjugate and substrate and check and judge by rapid color development Problem description: Low readingsPossible causes and corresponding countermeasures: 1.The microplate reader is not set up correctlyCheck wavelength and filter settings on the microplate reader/Turn on the ELISA reader in advance to preheat Problem description: Large coefficient of variationPossible causes and corresponding countermeasures: 1.Incorrect addition of liquidCheck the filling status Problem description: High background valuePossible causes and corresponding countermeasures: 1.The working concentration of the detection antibody is too highUse the recommended dilution factor 2.Incomplete washing of ELISA plateEnsure that each step is cleaned completely

doi: 10.1038/nrendo.2015.28 73 RebagliatoMMurciaMEspadaMlvarez-PedrerolMBolmarFVioqueJet al

Lan et al., 2012