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Materials and methods Post-mortem brain samples Frozen post-mortem tissues were obtained from the NICHD Brain and Tissue Bank for Developmental Disorders at the University of Maryland, Baltimore, MD, USA, and from the Autism Tissue Program at the Harvard Brain Tissue Resource Center, Belmont, MA, USA

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Mass spectrometry infrastructure in this work was partly supported by AMED-CREST (to Y.K.), and Platform for Drug Discovery, Informatics, and Structural Life Science from the ministry of Education, Culture, Sports, Science and Technology, Japan (T.K.)

Cells in 10-cm dishes were washed twice with 3 ml PBS and harvested with 1 ml trypsin, and the cell pellets (obtained by centrifuging at 300 g for 5 min at 4C) were washed twice with PBS