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Real TimePCR and melting curve analysis were done as described before 58

Curcumin nanoparticles: physicochemical fabrication, characterization, antioxidant, enzyme inhibition, molecular docking and simulation studies

For example, solid microneedles use a poke-and-patch strategy, whereas dissolving microneedles directly release encapsulated drugs upon dissolution within the skin (He et al., 2019)