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Effects of prebiotic galacto-oligosaccharide on postoperative cognitive dysfunction and neuroinflammation through targeting of the gut-brain axis

Cell culture HepG2-C3 cells (ATCC) were cultured as recommended in Minimum Essential Medium (MEM) supplemented with 10% fetal calf serum (FCS) and 2 mM glutamine, 1 mM sodium pyruvate, 1% non-essential amino acids, 100 units/mL of penicillin and 100 g/mL of streptomycin, in a 5% CO 2 humidified atmosphere at 37C