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Cells were grown in RPMI 1640 medium containing 10 % fetal bovine serum (Hyclone, USA) in a 37C humidified incubator containing 5 % CO 2 and 95 % air

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This means quality varies dramatically between sources, and poor-quality product is one of the most common reasons for protocol failure

Its more functional than flashy, and feels like a product designed for performance rather than luxury, she continued

The rate of weight loss remained consistent throughout the study period