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doi: 10.22074/cellj.2016.4867 70

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However, glutathione levels decrease as you age, and natural production can also be slowed because of disease, medications, stress, trauma, infections, a poor diet, pollution, and radiation

Cell signaling : H2O2, a necessary evil for cell signaling

To assess the dynamic recovery of the glutathione redox state after oxidative shock 50 l 200 mM diamide was added to 450 l cell suspension and incubated for 5 min at 30C with constant shaking at 400 r.p.m