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To test the effect of reducing agents on an Azo-tagged POI, 90 L mScarlet- Strep -GG-Pap was mixed with 10 L of 100 mM reduced L-glutathione (GSH), 50 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP) or 100 mM NADPH tetrasodium salt, each dissolved in chromatography buffer B