dietary cysteine and glutathione GNC N-Acetyl-L-Cysteine NAC 600mg, Boosts Levels of Glutathione, 120 Capsules Principal cysteine pathways in cellular

Dried samples were reconstituted in 2-propanol/MeCN/H 2 O (4:3:1, v/v/v) for LC-MS analysis

O 2 is the conjugate base of a weak acid HOO (de Grey 2002) Since superoxide does not react with DNA directly, in the context of DNA damage is of important dismutation reaction of superoxide radical Although the concentration of hydroperoxyl radical (HOO ) is low, this reaction is significantly faster than the uncatalyzed reaction involving two molecules of superoxide radical The superoxide anion radicals can be formed in biological systems as a consequence of thiol-mediated repair of radicals (R ) by thiols (RSH), resulting in the formation of thiyl radicals (RS ) (Valko et al

Redox Signal 99 KimJ

Aug 2007;98(2):392-396

For gene silencing with siCTRL or siATF4, the cells were transfected for 48 h with Lipofectamine TM RNAiMAX Transfection Reagent according to the manufacturers protocol