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Co-immunoprecipitation (Co-IP) After treatment with the indicated compounds, cells were harvested with 50 l of IP lysis buffer (50 mM Tris HCl, pH 7.5, 100 mM NaCl, 2 mM EDTA, 5% glycerol, 0.5% Triton X-100) supplemented with protease/phosphorylase inhibitors for each well of the 6-well plate and lysis was performed by incubation for 30 min on ice

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Therefore, future research should focus on creating a comprehensive framework that integrates mechanistic elucidation, target validation, and intervention optimization