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Cell culture and peptide handling The HCC line HepG2 (American Type Culture Collection (ATCC) Manassas, VA, USA) was maintained in Dulbeccos modified Eagles media (DMEM) with 4.5 g/l glucose (Corning, Corning, NY), 10% fetal bovine serum (Corning), and 100 U/ml penicillin and streptomycin (Gibco)

Zinc, calcium, iron, selenium, iodine, methyl-cobalamin, vitamin A, thiamine, pantothenic acid and niacin are the most common nutrient deficiencies in individuals with ASD [19]

Therefore, cell death can be harnessed for ceasing seizures and epileptogenesis

Regular tyrosine struggles to cross the blood-brain barrier efficiently, while NALT delivers this critical amino acid to support peak cognition when you need it most

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