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For caspase-4-GSDMD-induced liposome leakage, 0.2 M recombinant human His-sumo-GSDMD was pretreated with indicated concentrations of MeCbl for 0.5 h, then incubated with 0.2 M active-caspase-4 for another 2 h, 200 M liposome was then added into the caspase-4-GSDMD reaction system and incubated at 37 C overnight for final fluorescence measurements

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Step 5: Gently Swirl to Ensure Complete Reconstitution After waiting, its time to ensure everythings dissolved