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When cardiolipin is reduced or lost, cytochrome c is free to move to the cytoplasmic membrane and initiate apoptosis

J Hepatol (2014) 61:136575

The accumulation of ROS is particularly detrimental in cardiac tissues, where it can disrupt calcium homeostasis, contributing to cardiac dysfunction and arrhythmias (79)

The supernatant (cytosolic fraction) was then collected and the organellar pellet washed twice and resuspended in 100 L of 1% Triton X-100 (VWR, 0694) in 50 mM Tris-HCl and 150 mM NaCl, pH 7.4 to lyse the mitochondrial membrane