acytl l carnitine and cancer

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Name: acytl l carnitine and cancer acetyl-L-carnitine as a supplement in acetyl l carnitine brain cancer
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doi: 10.1016/j.jbior.2020.100741 60 SestiliP.StocchiV

10.1111/exd.13548 5 Carvajal-YepesM.HimmelsbachK.SchaedlerS.PloenD.KrauseJ.LudwigL.et al (2011)

sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml