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Inhibitory effect of black cumin ( Nigella sativa ) seed essential oil on Fusobacterium nucleatum L-methionine--lyase (L-methioninase) activity

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Analysis of l-lysine, 5AVA and the diamine cadaverine was performed by an automatic pre-column derivatization with ortho -phthaldialdehyde (OPA) and separated on a reversed phase HPLC using pre- and main column (LiChrospher 100 RP8 EC-5, 125 4.6 mm, CS Chromatographie Service GmbH) with l-asparagine as internal standard (Schneider and Wendisch, 2010)