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Marcel Monnier and Lucien Hsli at Basel had reported, in cross-circulation experiments dating back to the 1960s, that low-frequency electrical stimulation of the intralaminar thalamic nuclei in donor rabbits produced a humoral factor that, when transferred to awake recipient rabbits via dialysed cerebral venous blood, induced an EEG pattern resembling slow-wave sleep

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We used the LABScreen Mixed kit (One Lambda Inc., Canoga Park, CA, USA), which simultaneously detects class I and class II antibodies and the SAB assays (Single Antigen kit, One Lambda) to identify HLA class I and class II specificities (22)