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10.1113/JP274229 39 PintoF.MoraesN.MarquesM.NetoH

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Select the chromatography method based on the difference in the properties of the target protein and the impurity protein: Ion Exchange Chromatography: Large difference in isoelectric points Hydrophobic Interaction Chromatography: Large differences in hydrophobicity Gel Filtration Chromatography: Large difference in molecular weight Multimodal Chromatography: Ion exchange and hydrophobic interaction chromatography alone cannot effectively separate Reversed-phase Chromatography: Generally not selected in protein purification, but can be selected in the purification of small molecule polypeptides Among them, ion exchange chromatography is convenient to operate and widely used