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H&E, Alcian blue, and Prussian blue staining were performed according to the staining kits protocol (Servicebio) after deparaffinization

Consistently, a complementary strategy showed that in aged mice, where IGF-I levels are decreased, cardiac overexpression of IGF-I significantly improved cardiomyocyte contractile function[283], attenuating oxidative stress-mediated protein damage, normalizing Ca 2+ homeostasis, reversing age-related alterations in the expression of pro-apoptotic proteins, and decreasing the apoptosis rate[284]

465 Oral administration of NR halts the progression of NAFLD through rescuing the NAD + reduction, reducing the total cholesterol and triglyceride levels, decreasing the AST level, and reversing Kupffer cells accumulated and inflammatory in aged group

Free radicals can damage cells and DNA if left unchecked, resulting in issues such as: Increased signs of aging (such as wrinkled or dry skin) Headaches Low energy levels Increased risk of illness Lack of mental clarity Joint pain Fatigue Antioxidants, such as glutathione, rid the body of free radicals before they can do too much harm

10.1002/adma.202305964 41 ChengA.-W.TanX.SunJ.-Y.GuC.-M.LiuC.GuoX