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Of course, 100 mg of protein is a lot for most applications so you may be fine with that yield and not care about losing some of your protein in the flow-through, depending on what the downstream application is for your protein

The analysis was conducted using SPSS software, version 16 (SPSS Institute, Chicago, IL, USA)

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Yet, limitations in the experimental studies, as mentioned above, and the absence of clinical investigations hinder a conclusive determination of the beneficial role of IL-22 in the context of MASH