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The samples were then centrifuged at 5000g at 4 C for 35 min, and 20 _ of the supernatants were analyzed by LC-MS/MS for quantitation of the remaining test article

It's common - especially after walking or standing for long periods - and it's often remedied by resting and elevating those tired "dogs." But if it persists, you likely need to see a doctor because swelling (also called edema) can be a red flag for a more serious underlying problem, such as heart failure, deep-vein thrombosis (a blood clot in a leg vein), kidney disease, or cirrhosis of the liver

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