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For comparison, we treated cells with tunicamycin (TUN, a widely-used ER stress inducer) which did not alter phospho-GCN2 (T899), but reduced the mobility of PERK, indicative of its increased phosphorylation (Fig

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Future studies identifying the cell types and mechanisms responsible for high protein nitration in the rostral corpus callosum will allow better understanding of the regional differences with powdered and pelleted cuprizone