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After 3 h, the cells were returned to a normal medium and incubated under normal conditions for 30 min

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In summary, protein lysates were prepared using Pierce IP Lysis/Wash Buffer containing protease and phosphatase inhibitor mixtures (Biosharp) and incubated with IP antibody overnight at 4 C

Comment: Many commenters expressed support for CMS' decision to not pursue a 340B claims indicator at this time and urged that CMS should not revisit the idea